Substrate specificity of tissue plasminogen activator and urokinase as determined with synthetic chromogenic substrates.

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Substrate specificity of tissue plasminogen activator and urokinase as determined with synthetic chromogenic substrates.

Three different synthetic chromogenic substrates )H-glutamylglycyl-L-arginine-p-nitroanilide (S-2227), pyro-glutamyl-glycyl-L-arginine-p-nitroanilide (S-2444), and H-D-isoleucyl-L-prolyl-L-arginine-p-nitroanilide (S-2288] were investigated for use in the measurement of plasminogen activator activity with high molecular weight urokinase (H-UK), low molecular weight urokinase (L-UK), and tissue p...

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Hepsin, a type II transmembrane serine protease, is strongly up-regulated in prostate cancer. Hepsin overexpression in a mouse prostate cancer model resulted in tumor progression and metastasis, associated with basement membrane disorganization. We investigated whether hepsin enzymatic activity was linked to the basement membrane defects by examining its ability to initiate the plasminogen/plas...

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Increased plasminogen activator (urokinase) in tissue culture after fibrin deposition.

Lysis of fibrin in tissue culture has been shown to be due to plasminogen activator identified immunologically as urokinase. The present study examines fibrinolytic events in culture, particularly mechanisms leading to increased urokinase levels and accelerated fibrinolysis. Deposition of fibrin on cells in culture was followed by a two- to six-fold increase in urokinase in the supernates and r...

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ژورنال

عنوان ژورنال: The Japanese Journal of Physiology

سال: 1983

ISSN: 1881-1396,0021-521X

DOI: 10.2170/jjphysiol.33.1031